proliferative phases  (Bio-Techne corporation)

Journal: Human Reproduction (Oxford, England)

Article Title: The role of small extracellular vesicle-miRNAs in endometriosis

doi: 10.1093/humrep/dead216

Figure Lengend Snippet: Studies investigating the differential expression of sEV-miRNA.

Article Snippet: 13 , Uterine aspirate fluid , Human samples: Endometriosis (stage III–IV, endometrioma, diagnosed via laparoscopy), n=22 Control (including simple ovarian cyst, uterine leiomyoma, tubal ligation), n=25 *Mentioned about that findings were regardless of secretory or proliferative phases in the results section but did not declare number of samples that were of proliferative or secretory. , Size exclusion chromatography exosome isolation kit (Echobiotech, Beijing, China) , NTA : Median size: 111.7 nm and the proportion of the main peak was 94.1%. Visualised via TEM . , WB : CD63, TSG101, and HSP70 positive. Calnexin negative. , Microarray analysis of miRNA from sEVs and endometrial tissues from uterine aspirate fluid, followed by qRT-PCR . sEV uptake experiments with JNK activator anisomycin by M0 macrophages followed by flow cytometry , WB, and MAPK phosphorylation antibody array . Transwell migration and invasion assay : Co-culture of endometrial tissues of control group and sEV-treated macrophages. Transfection of M0 macrophages with hsa-miR-210-3p mimic/inhibitor/corresponding NC followed by qRT-PCR and flow cytometry . , ↑ hsa-miR-210-3p Regardless of secretory or proliferative phases , Jiang et al. (2022) .

Techniques: Expressing, Isolation, Concentration Assay, Western Blot, Microarray, Quantitative RT-PCR, Mouse Assay, Immunohistochemistry, Extraction, Transfection, Transmission Electron Microscopy, Sequencing, Flow Cytometry, Reporter Gene Assay, Zeta Potential Analyzer, Cell Culture, In Situ, Hybridization, Wound Healing Assay, Marker, Ligation, Migration, Size-exclusion Chromatography, Ab Array, Invasion Assay